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Employing the histogram matching approach, the sediment regime alteration of the upper Yangtze River was assessed and its possible causes and environmental influences were discussed. Daily sediment load and flow data from 1950 to 2008 were collected from Yichang hydrometric station. The annual sediment load series was segmented by heuristic segmentation algorithm in 1986 and 2003, and the multi-year mean values of the three phases were 5.28� ×� 10⁸, 3.89� ×� 10⁸ and 0.57� ×� 10⁸� t, respectively. One change point was found by cross wavelet transform in 2003 when the cross wavelet power became insignificant, which indicates the interruption of the common annual cycle of the sediment load and flow series. The sediment regime from 1986 to 2002 was altered by a total of 41� % compared with the regime from 1950 to 1985. It may be mainly attributed to numerous dams constructed in the tributaries, the Gezhouba Dam constructed in the mainstream, and land use and land cover change, e.g., the increased grassland with a coverage density larger than 50� %. The sediment regime alteration after 2003, assessed to be a total of 83� %, may be mainly attributed to the Three Gorges Reservoir. It breaks the natural process of sediment transport, reduces sediment concentration and releases clear water to the downstream which may result in some environmental problems, e.g., limited nutrients availability, river bed erosion, benthic habitat destruction, etc. The assessment of sediment regime alteration may provide some references for the environmental management and conservation of the Yangtze River.