The aim of this study was to verify the accuracy of the pedigree data of Old Kladruber dam lines using mtDNA analysis. The upstream part of the mtDNA D-loop hypervariable region (384 bp, nt15450 - nt15834) was sequenced. DNA was extracted from deep-frozen whole blood and fresh saliva samples of 170 Old Kladruber horses representing 23 dam lines from Kladruby n/L stud and some private farms.

This Regulation amends Annex 1 of the Regulation establishing vulnerable areas and the use and storage of fertilizers and manure fertilizers, crop rotation and implementation of erosion-control measures in these areas. This Annex specifies vulnerable areas which are geographically defined by the cadastral territory of the Czech Republic.

Implements: Water Act. (2001-06-28)
Amends: Regulation establishing vulnerable areas and the use and storage of fertilizers and manure fertilizers, crop rotation and implementation of erosion-control measures in these areas. (2003-03-03)

This Decree of the Ministry of Agriculture lays down some amendments and addenda to Decree on agrochemical examination of agricultural lands and assessment of soil properties of forest lands. In particular the replaced Annex 1 provides the list of hazardous elements and hazardous substances monitored in agrochemical testing of agricultural land and parameters monitored during soil analysis. Annex 2 describes methods used for chemical, physical and microbiological analysis of agricultural land and forest land.

This Government Regulation lays down, in accordance with European Union legislation, conditions of subsidies for afforestation of agricultural land. Subsidies are granted under the following measures: (a) establishment of forest stands; (b) care of forests for five calendar years starting in the year following the year when the forest was established; (c) cessation of agricultural production on wooded land for a period of ten calendar years starting in the year following the year when the forest was established.

This Decree of the Ministry of Environment lays down detailed rules for the quality protection of agricultural land. The preventive values of hazardous substances in the agricultural land are set out in Annex 1. The indicative values of hazardous substances in agricultural land are set out in Annex 2. Determination of the contents of hazardous substances in agricultural land shall be carried out using the procedures is specified in Annex 3. The sampling procedure for determination of contents of hazardous substances in agricultural land is set out in Annex 4.