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Land use and land cover change (LULC) is an essential component for the monitoring environmental change and managing natural resources in areas of high natural and cultural biodiversity, such as the Amazon biome. This study was conducted in in the northern Amazon of Ecuador, specifically in the Diversity and Life Zone (DLZ) of the Yasuní Biosphere Reserve (YBR). The general aim was to investigate the territorial dynamics of land use/land cover changes to support policies for environmental and sociocultural protection in the DLZ. Specific objectives included (i) mapping LULC spatial and temporal dynamics in the DLZ in the period from 1999 to 2018, (ii) identifying sensitive LULC hotspots within the DLZ, and (iii) defining the possible policy implications for sustainable land use in the DLZ. Multitemporal satellite imagery from the Landsat series was used to map changes in LULC, which were divided into three-time stages (1999–2009, 2009–2018, 1999–2018). We adopted open-access Landsat images downloaded from the United States Geological Survey (USGS). The processes for assessing LULC in the DLZ included (1) data collection and analysis, (2) data processing for remote sensing, (3) thematic land cover, and (4) homogenization and vectorization of images. The results showed that in the period 1999–2018, most of the uses and land cover were transformed into pastures in the DLZ. Therefore, it is important to improve territorial planning, to avoid conflicts between indigenous populations, migrant settlers, and uncontacted indigenous populations that live in the DLZ, within the YBR.
