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There is increasing interest in Zimbabwe in developing a biofuels industry based on the production of biodiesel using Jatropha as the main feedstock. This has led to the introduction of Jatropha as a commercial energy crop in the country. There are plans to grow 1220 km2 of Jatropha which will supply about 365,000 t of seed. This will provide about 110 dam3 of biodiesel required to achieve a blending level of 10% with petro-diesel. The availability and suitability of land for the production of Jatropha cannot be taken for granted, particularly given the fact that the concept and practice of production of feedstocks for biofuels remain contested on the threat they pose to food security. Determining the land that is potentially available for biofuels is a non-trivial task. A multiplicity of factors needs to be considered. It is important to determine the spatial extent of areas with suitable growth conditions for Jatropha. The interaction of soil type and land use is an important interface in agriculture. Added to this is the need to balance food, fodder and fuel supply in land use planning. This paper attempts to assess the availability and suitability of various land types as well as agro-ecological conditions for the production of Jatropha in Zimbabwe.